RNA catalysis.
نویسنده
چکیده
Our understanding of the relationship between the structure of RNA and its catalytic activity has advanced significantly in the past year. These advances include time-resolved crystallographic studies on the hammerhead ribozyme, as well as new structures of a group I intron, a lead(II)-cleavage ribozyme, a hepatitis delta virus ribozyme, and components of the spliceosome machinery and the peptidyl transferase center of the ribosome and, most significantly, the structure of the ribosome itself.
منابع مشابه
The origins of RNA catalysis in ribozymes.
The discovery of RNA catalysis provided a paradigm shift in biology, insight into the evolution of life on the planet and a challenge to understand its mechanistic origins. RNA has limited catalytic resources that must be used to maximal effect. Consequently, RNA catalysis tends to be multifactorial, with several processes contributing to an overall significant enhancement of reaction rate. The...
متن کاملMechanistic aspects of enzymatic catalysis: lessons from comparison of RNA and protein enzymes.
A classic approach in biology, both organismal and cellular, is to compare morphologies in order to glean structural and functional commonalities. The comparative approach has also proven valuable on a molecular level. For example, phylogenetic comparisons of RNA sequences have led to determination of conserved secondary and even tertiary structures, and comparisons of protein structures have l...
متن کاملKinetics of RNA Degradation by Specific Base Catalysis of Transesterification Involving the 2′-Hydroxyl Group
A detailed understanding of the susceptibility of RNA phosphodiesters to specific base-catalyzed cleavage is necessary to approximate the stability of RNA under various conditions. In addition, quantifying the rate enhancements that can be produced exclusively by this common cleavage mechanism is needed to fully interpret the mechanisms employed by ribonucleases and by RNA-cleaving ribozymes. C...
متن کاملRibozymes: structure and mechanism in RNA catalysis.
The hammerhead RNA is a small catalytic RNA found in a number of RNA virus genomes and virus-like RNAs. The recently determined crystal structures of hammerhead ribozymes reveal how a small RNA motif can fold up into a conformation suitable for mediating RNA cleavage.
متن کاملReviewTwo Decades of RNA Catalysis
enzymology, the sensitive nature of RNA structure Twenty years have passed since the initial discovery brings new complexities to these experiments. Considof catalytic RNA. Although initial discoveries of riboeration of RNA catalysis in general [7, 8] and more spezymes (RNA enzymes) involved phosphoryl transfer cific discussions of ribozyme structure and function [9, reactions on RNA substrates...
متن کاملHammerhead ribozymes: true metal or nucleobase catalysis? Where is the catalytic power from?
The hammerhead ribozyme was first considered as a metalloenzyme despite persistent inconsistencies between structural and functional data. In the last decade, metal ions were confirmed as catalysts in self-splicing ribozymes but displaced by nucleobases in self-cleaving ribozymes. However, a model of catalysis just relying on nucleobases as catalysts does not fully fit some recent data. Gatheri...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Current opinion in structural biology
دوره 8 6 شماره
صفحات -
تاریخ انتشار 1998